James VI and I King of Scotland from 1566 to 1625, King of England and Ireland from 1603 to 1625 (1566–1625) Parents Mary, Queen of Scots Henry Stuart, Lord Darnley Children Charles I of England Mary Stuart unnamed son Stuart unnamed son Stuart Jacob Kettler Sophia of England Henry Frederick, Prince of Wales Elizabeth Stuart, Queen of Bohemia Margaret Stuart Robert Stuart, Duke of Kintyre and Lorne Siblings George Stewart, 9th Seigneur d'Aubigny Partners Anne of Denmark