Maria Manuela, Princess of Portugal Intanta of Portugal, Princess of Asturias, Duchess of Milan (1527–1545) Parents Catherine of Austria, Queen of Portugal John III of Portugal Children Carlos, Prince of Asturias Siblings Afonso, Prince of Portugal Philip, Prince of Portugal João Manuel, Prince of Portugal Manuel, Prince of Portugal António, Infante de Portugal Eduardo de Portugal Dionisio de Portugal y Austria Partners Philip II of Spain
