Juana I of Castile Queen of Castile from 1504 and of Aragon from 1516 (1479–1555) Parents Isabella I of Castile Ferdinand II of Aragon Children Eleanor of Austria Ferdinand I, Holy Roman Emperor Catherine of Austria, Queen of Portugal Charles V Mary of Hungary Isabella of Austria Siblings John, Prince of Asturias Isabel of Aragon Catherine of Aragon Alonso de Estrada John of Aragon, Prince of Girona Maria of Aragon Alonso de Aragón Juana de Aragón Ruiz de Ivorra María Esperanza de Aragón Partners Philip I of Castile